Preparation of YAC end fragments from the whitehead/MIT mouse YAC library pRML vectors.
نویسندگان
چکیده
منابع مشابه
Vector-hexamer PCR isolation of all insert ends from a YAC contig of the mouse Igh locus.
We have developed a simple PCR strategy, termed vector-hexamer PCR, that is unique in its ability to easily recover every insert end from large insert clones in YAC and BAC vectors. We used this method to amplify and isolate all insert ends from a YAC contig covering the mouse Igh locus. Seventy-seven ends were amplified and sequenced from 36 YAC clones from four libraries in the pYAC4 vector. ...
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عنوان ژورنال:
- BioTechniques
دوره 26 3 شماره
صفحات -
تاریخ انتشار 1999